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Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization

Guido Werner1, Melanie Bartel, Nele Wellinghausen

  • 1Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Kochstr. 8, D-89081 Ulm, Germany.

Journal of Clinical Microbiology
|May 4, 2007
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...