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Updated: Jul 15, 2026
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
Published on: September 9, 2015
Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility
A Espinel-Ingroff1, A Fothergill, M Ghannoum
1VCU Medical Center, 1101 E. Marshall Street, Sanger Hall, Room 7-049, P.O. Box 980049, Richmond, VA 23298-0049, USA. avingrof@vcu.edu
Abstract:
The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and minimum effective concentrations [MECs]) for evaluating anidulafungin against molds. Anidulafungin MICs (>or=50% inhibition) and MECs (morphological hyphal changes) were determined for seven Aspergillus isolates (four species) and one isolate each of Fusarium moniliforme, Fusarium solani, and Paecilomyces variotii and for two Scedosporium apiospermum isolates. The inter- and intralaboratory reproducibilities of 10 replicate tests performed in each laboratory on 10 different days for each isolate was 100% at 24 h (MECs,
Insights
This study assessed anidulafungin antifungal susceptibility testing for filamentous fungi. Optimal conditions were identified, showing high reproducibility for molds like Aspergillus, Fusarium, and Scedosporium.
Area of Science:
- Medical Mycology
- Antimicrobial Susceptibility Testing
- Clinical Microbiology
Background:
- Current Clinical and Laboratory Standards Institute (CLSI) M38-A guidelines lack echinodin recommendations.
- Antifungal susceptibility testing (AFST) is crucial for treating mold infections.
Purpose of the Study:
- To evaluate the inter- and intralaboratory reproducibility of anidulafungin AFST against filamentous fungi.
- To determine optimal reading times and endpoints for anidulafungin susceptibility testing.
Main Methods:
- A multicenter study involving eight laboratories.
- Testing anidulafungin against Aspergillus, Fusarium, Paecilomyces, and Scedosporium isolates.
- Assessing minimum inhibitory concentrations (MICs) and minimum effective concentrations (MECs) at various time points (24, 48, 72 hours).
Main Results:
- 100% reproducibility for anidulafungin MECs at 24 hours for Aspergillus and Paecilomyces.
- High reproducibility (94-97.5%) for Fusarium and Scedosporium isolates at 48 or 72 hours.
- Established optimal testing conditions for anidulafungin against key mold species.
Conclusions:
- The study identified reliable testing conditions for anidulafungin against molds.
- Recommendations for incorporating these findings into future CLSI M38 guidelines are warranted.
- Standardized testing will improve clinical management of fungal infections.
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