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Cyclic AMP in developing muscle of the rhesus monkey: effect of prostaglandin E
Abstract:
Cyclic AMP levels, measured by a competitive protein-binding assay and by a prelabeling technique with adenine-14 C, were determined on fetal, infant, and adult heart, diaphragm, and skeletal muscle of the rhesus monkey (Macaca mulatta); the effect of PGE on cyclic AMP accumulation was also studied. The levels of cyclic AMP were higher in the 3 types of muscle from 150-day fetuses (91% of term) than in adult muscles and even higher at 78-100 days fetal age, and histological evidence indicated that by 65 days gestation, the majority of the rhesus skeletal muscle cells are in the myotube stage. These data agree with the observation in cell cultures that a decrease in cyclic AMP is correlated with fusion of myoblasts to form myotubes. PGE (2.8 MUM) stimulated cyclic AMP accumulation in all the fetal series, even in the earliest series (47% of term), as well as in all the adult series.
Insights
Cyclic AMP levels are higher in fetal rhesus monkey muscles than adult muscles. Prostaglandin E stimulated cyclic AMP accumulation across all fetal and adult muscle types studied.
Area of Science:
- Biochemistry
- Developmental Biology
- Muscle Physiology
Background:
- Cyclic adenosine monophosphate (cAMP) plays a crucial role in cellular signaling and muscle development.
- Previous research suggests a correlation between decreased cAMP levels and myoblast fusion in cell cultures.
Purpose of the Study:
- To investigate the developmental changes in cyclic AMP (cAMP) levels in various rhesus monkey muscles.
- To examine the effect of Prostaglandin E (PGE) on cAMP accumulation in fetal and adult muscle tissues.
Main Methods:
- Measurement of cAMP levels using competitive protein-binding assay and adenine-14C prelabeling.
- Histological examination of rhesus monkey skeletal muscle development.
- In vitro stimulation of muscle tissues with Prostaglandin E (PGE).
Main Results:
- Fetal rhesus monkey muscles exhibited significantly higher cAMP levels compared to adult muscles, with levels increasing at earlier gestational ages.
- Histological data indicated that a majority of skeletal muscle cells reach the myotube stage by 65 days gestation.
- Prostaglandin E (2.8 µM) effectively stimulated cAMP accumulation in all tested fetal and adult muscle samples.
Conclusions:
- Elevated cAMP levels during fetal development may be associated with muscle maturation and myogenesis.
- The findings support the inverse relationship between cAMP levels and myoblast fusion observed in cell culture studies.
- Prostaglandin E demonstrates a consistent ability to enhance cAMP accumulation in both developing and mature rhesus monkey muscles.