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Related Experiment Videos

Fluorescence spectrometer-on-a-fluidic-chip.

Oliver Schmidt1, Michael Bassler, Peter Kiesel

  • 1Palo Alto Research Center Inc. (PARC), 3333 Coyote Hill Rd, Palo Alto, CA 94304, USA.

Lab on a Chip
|May 4, 2007
PubMed
Summary
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A compact spectrometer using a linear variable band-pass filter and CMOS camera analyzes light spectra. Integrated fluidics enable fluorescence analysis of moving objects in real-time.

Area of Science:

  • Optics and Photonics
  • Microfluidics
  • Spectroscopy

Background:

  • Traditional spectrometers can be bulky and expensive.
  • Real-time analysis of moving samples presents challenges in spectral detection.

Purpose of the Study:

  • To develop a miniaturized spectrometer for spectral analysis.
  • To integrate a fluidic system for analyzing fluorescence from moving objects.

Main Methods:

  • A chip-size spectrometer was created by integrating a linear variable band-pass filter with a CMOS camera.
  • Spectral information was converted into a spatially dependent signal for camera analysis.
  • A fluidic platform was integrated for real-time fluorescence detection of moving targets within an anti-resonant waveguide.

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Main Results:

  • The integrated system successfully converted spectral data into spatial signals.
  • Fluorescence spectra of moving objects were recorded as they traversed the detection area.
  • Demonstrated feasibility of chip-scale spectral analysis for dynamic fluidic samples.

Conclusions:

  • A compact, integrated spectrometer system has been successfully developed.
  • The device enables real-time spectral analysis of fluorescence from moving objects in microfluidic systems.
  • This technology offers potential for portable and high-throughput spectral sensing applications.