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Updated: Jul 15, 2026

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
06:53

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Published on: May 17, 2020

Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis

Hsin-Yuan Tan1, Yen Sun, Wen Lo

  • 1Chang Gung University, College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan, Taiwan.

Journal of Biomedical Optics
|May 5, 2007
PubMed

Insights

Multiphoton microscopy visualizes human corneal infections ex-vivo by imaging cellular and collagen changes. This technique identifies structural alterations without histology, aiding potential clinical diagnosis of corneal diseases.

Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Microscopy

Background:

  • Corneal infections cause significant structural changes.
  • Accurate visualization is crucial for diagnosis and treatment.
  • Current diagnostic methods may require invasive procedures.

Purpose of the Study:

  • To demonstrate multiphoton fluorescence and second harmonic generation (SHG) microscopy for ex-vivo human corneal visualization.
  • To assess the capability of these imaging techniques in identifying infectious processes.
  • To evaluate the potential for clinical application in diagnosing corneal infections.

Main Methods:

  • Utilized multiphoton fluorescence microscopy to visualize cellular components.
  • Employed second harmonic generation (SHG) microscopy to image collagenous structures.
  • Applied these techniques for ex-vivo analysis of human corneal samples with infectious processes.

Main Results:

  • Multiphoton microscopy effectively visualized structural alterations in corneal cells and collagen.
  • Fluorescence imaging identified cellular changes, while SHG imaging revealed collagenous component alterations.
  • Pathogens exhibiting fluorescence were detectable within turbid corneal specimens.
  • The technique successfully identified structural alterations without the need for histological processing.

Conclusions:

  • Multiphoton microscopy is effective for ex-vivo visualization of corneal infections.
  • The combined fluorescence and SHG imaging modalities provide comprehensive structural information.
  • Further development could lead to a valuable clinical diagnostic and monitoring tool for corneal infections.

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