Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Operando</i> TEM study of working TiO<sub>2</sub> supported Au and Au-Cu catalysts during butadiene hydrogenation.

Nanoscale·2026

Same author

Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.

Nature methods·2026

Same author

A common signature of brain metastases among patients with breast cancer, melanoma, and lymphoma.

Neuro-oncology advances·2026

Same author

Help-Seeking and Substance Use Among Police Staff After the 2018 Strasbourg Christmas Market Attack.

American journal of industrial medicine·2026

Same author

The hidden burden of narcolepsy type 1: Discordance in psychobehavioral symptoms between patient self-reports and reports from close family members and friends.

Sleep medicine·2026

Same author

General and Toxicologic Aspects of Occupational Fatalities in the Metropolitan Area of Lyon From 2000 to 2020, a Retrospective Study.

La Medicina del lavoro·2026

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

Mirror slides for high-sensitivity cell and tissue fluorescence imaging.

Eric Le Moal¹, Emmanuel Fort, Sandrine Léve Que-Fort

¹Université Denis Diderot - Paris VII, UMR 7162 CNRS, Laboratoire Matériaux et Phénomènes Quantiques, Paris Cedex, France. eric.lemoal@espci.fr

Journal of Biomedical Optics

|May 5, 2007

Summary

Mirror slides enhance fluorescence microscopy signals for better cell and tissue imaging. This breakthrough improves high-sensitivity analysis and high-throughput screening in life sciences.

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

3D Flipwell Engineering for Developing Asynchronous Systems for Toxicologic and Immunomodulatory Therapies in Bacterial, Gut, and Immune Cells

3D Flipwell Engineering for Developing Asynchronous Systems for Toxicologic and Immunomodulatory Therapies in Bacterial, Gut, and Immune Cells

Published on: October 17, 2025

Related Experiment Videos

Last Updated: Jul 15, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

3D Flipwell Engineering for Developing Asynchronous Systems for Toxicologic and Immunomodulatory Therapies in Bacterial, Gut, and Immune Cells

3D Flipwell Engineering for Developing Asynchronous Systems for Toxicologic and Immunomodulatory Therapies in Bacterial, Gut, and Immune Cells

Published on: October 17, 2025

Area of Science:

Life Sciences
Microscopy
Biotechnology

Background:

Fluorescence microscopy is a primary technique in life sciences.
Standard microscopy faces limitations in signal strength and field of view.

Purpose of the Study:

To develop and utilize mirror slides for enhanced fluorescence signal.
To improve imaging capabilities for routine analysis and high-throughput screening.

Main Methods:

Development of novel mirror slides.
Integration of mirror slides with standard air microscope objectives.
Application to cell and tissue samples.

Main Results:

Significant enhancement of fluorescence signal achieved.
High-sensitivity imaging enabled.
Wide field of observation and large depth of focus demonstrated.

Conclusions:

Mirror slides offer a breakthrough for fluorescence microscopy.
The technique is suitable for routine analysis and high-throughput screening.
Enhanced imaging quality benefits cell and tissue sample analysis.