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Related Experiment Videos

The rat GPRC6A: cloning and characterization.

Petrine Wellendorph1, Nicole Burhenne, Bolette Christiansen

  • 1Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. pw@farma.ku.dk

Gene
|May 5, 2007
PubMed
Summary
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Researchers characterized the rat GPRC6A, a G protein-coupled receptor, identifying l-ornithine as its most potent amino acid agonist. This study highlights the receptor

Area of Science:

  • Pharmacology
  • Molecular Biology
  • Genomics

Background:

  • G protein-coupled receptors (GPCRs) are crucial cell surface receptors involved in numerous physiological processes.
  • GPRC6A, a member of the GPCR family C, has an incompletely understood biological function.
  • Previous studies identified GPRC6A in humans and mice as a receptor for various l-alpha-amino acids.

Purpose of the Study:

  • To clone, express, and functionally characterize the rat orthologue of GPRC6A (rGPRC6A).
  • To investigate the tissue distribution and protein characteristics of rGPRC6A.
  • To determine the ligand profile and pharmacological properties of rGPRC6A.

Main Methods:

  • Full-length cDNA amplification from rat taste tissue for rGPRC6A cloning.
  • Reverse transcriptase polymerase chain reaction (RT-PCR) for mRNA expression analysis.

Related Experiment Videos

  • Cell-based inositol phosphate turnover assays using co-expression systems to assess receptor activity.

    • Main Results:

    • Rat GPRC6A (rGPRC6A) was successfully cloned and its identity confirmed at genomic and protein levels.
    • rGPRC6A mRNA showed wide but weak distribution, with high expression in the soft palate.
    • l-ornithine emerged as the most potent agonist (EC50 = 264 microM), modulated positively by Mg2+.

    Conclusions:

    • The study provides comprehensive characterization of rGPRC6A, confirming evolutionary conservation.
    • Findings indicate significant physiological roles and potential pharmacological applications for GPRC6A.
    • l-ornithine and Mg2+ represent key modulators of rGPRC6A activity.