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NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
A novel amperometric biosensor based on ZnO:Co nanoclusters for biosensing glucose.
1School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798, Singapore. zzsg@hotmail.com
Biosensors & Bioelectronics
|May 5, 2007
Summary
This study introduces a new amperometric glucose biosensor using zinc oxide-cobalt (ZnO:Co) nanoclusters. This novel biosensor demonstrates high sensitivity and a low detection limit for glucose detection.
Area of Science:
- Materials Science
- Nanotechnology
- Electrochemistry
Background:
- Biosensors are crucial for detecting biological analytes like glucose.
- Developing sensitive and stable biosensor platforms remains a challenge.
- Nanomaterials offer unique properties for biosensor applications.
Purpose of the Study:
- To synthesize ZnO:Co nanoclusters for biosensor construction.
- To develop a novel amperometric glucose biosensor using these nanoclusters.
- To evaluate the performance of the constructed glucose biosensor.
Main Methods:
- ZnO:Co nanoclusters synthesized via nanocluster-beam deposition (5 nm average size, porous structure).
- Glucose oxidase immobilized onto ZnO:Co nanocluster-assembled thin films using Nafion-assisted cross-linking.
- Amperometric detection of glucose.
Main Results:
- The ZnO:Co nanocluster-based biosensor exhibited high sensitivity (13.3 microA/mA cm2).
- A low detection limit of 20 microM (S/N=3) was achieved.
- High enzyme affinity for glucose (apparent Michaelis-Menten constant of 21 mM) was observed.
Conclusions:
- ZnO:Co nanocluster-assembled thin films are effective platforms for enzyme immobilization.
- The nanoporous structure and nanocrystallites enhance biosensor performance.
- This novel glucose biosensor shows significant potential for practical applications.
