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Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
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Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

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Related Experiment Video

Updated: Jul 15, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

New molecular players in capacitative Ca2+ entry.

James W Putney1

  • 1National Institute of Environmental Health Sciences - NIH, PO Box 12233, Research Triangle Park, NC 27709, USA. putney@niehs.nih.gov

Journal of Cell Science
|May 5, 2007
PubMed
Summary

Capacitative calcium entry (CCE) is activated by Stim1, an ER calcium sensor. Stim1 signals to Orai proteins in the plasma membrane, which form the pores of store-operated calcium channels.

Area of Science:

  • Cell Biology
  • Molecular Physiology
  • Calcium Signaling

Background:

  • Capacitative calcium entry (CCE) links intracellular calcium store depletion to plasma membrane calcium channel activation.
  • For twenty years, the mechanisms and components of CCE, including Transient Receptor Potential (TRP) channels, have been investigated.
  • Recent discoveries have identified key molecular players in CCE.

Purpose of the Study:

  • To elucidate the roles of Stim1 and Orai proteins in the signaling and permeation of store-operated calcium channels.
  • To understand the spatial dynamics of Stim1 during CCE.
  • To confirm Orai proteins as the pore-forming subunits of store-operated channels.

Main Methods:

  • The study focuses on the molecular mechanisms of capacitative calcium entry.

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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell
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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Published on: October 22, 2014

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Last Updated: Jul 15, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell
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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Published on: October 22, 2014

  • Investigates the function and localization of Stim1 and Orai proteins.
  • Utilizes concepts from cell biology and molecular physiology to analyze calcium signaling pathways.

    • Main Results:

    • Stim1 acts as an endoplasmic reticulum calcium sensor.
    • Stim1 redistributes within the ER to approach the plasma membrane but does not translocate into it.
    • Stim1 directly signals to Orai proteins, which are identified as the pore-forming subunits of store-operated channels.

    Conclusions:

    • Stim1 and Orai proteins are crucial components of the store-operated calcium channel complex.
    • Stim1's redistribution within the ER is a key signaling event for channel activation.
    • Orai proteins form the actual calcium-permeable pores of these channels, validating their role in CCE.