Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

A Versatile Method of Patterning Proteins and Cells

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Template-directed patterning of polymers and biomaterials.

Amol Chandekar¹, Sandip K Sengupta, James E Whitten

¹Department of Chemistry and Center for High-Rate Nanomanufacturing, University of Massachusetts Lowell, Lowell, Massachusetts 01854-5047, USA.

Microscopy Research and Technique

|May 8, 2007

Summary

This study introduces a new surface patterning technique using microcontact printing to create hydrophilic and hydrophobic regions. This method allows for the precise fabrication of synthetic and biological polymer patterns on gold surfaces.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Open-Air Plasma Treatment to Enhance Nanoparticle Adhesion on Nylon- and Cotton-Based Fabrics for Chemical Protection.

ACS applied materials & interfaces·2026

Same author

Adsorption of l-Cysteine and Cysteamine on Zinc Oxide Nanoparticles.

Langmuir : the ACS journal of surfaces and colloids·2024

Same author

Management of the Axilla in Early-Stage Breast Cancer: Ontario Health (Cancer Care Ontario) and ASCO Guideline.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2021

Same author

Thiol adsorption on metal oxide nanoparticles.

Physical chemistry chemical physics : PCCP·2021

Same author

An epigenetic increase in mitochondrial fission by MiD49 and MiD51 regulates the cell cycle in cancer: Diagnostic and therapeutic implications.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2020

Same author

Novel prognostic and predictive microRNA targets for triple-negative breast cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2018

Area of Science:

Materials Science
Surface Chemistry
Biotechnology

Background:

Surface patterning is crucial for controlling material properties and biological interactions.
Existing methods often lack precision or versatility in patterning diverse materials.

Purpose of the Study:

To develop a novel, versatile method for patterning surfaces with synthetic and biological polymers.
To demonstrate the fabrication of micron-scale polymer patterns using a tailored surface template.

Main Methods:

Microcontact printing was used to create a gold surface with distinct hydrophilic (16-mercaptohexadecanoic acid) and hydrophobic (perfluorinated thiol) regions.
Hydrophilic polymers and biomaterials were patterned onto the functionalized surface via spin-coating or drop-casting.
Atomic force microscopy (AFM), scanning electron microscopy (SEM), and fluorescence microscopy were employed for characterization.

More Related Videos

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Published on: March 7, 2014

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Related Experiment Videos

Last Updated: Jul 15, 2026

A Versatile Method of Patterning Proteins and Cells

A Versatile Method of Patterning Proteins and Cells

Published on: February 26, 2017

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Cell Patterning on Photolithographically Defined Parylene-C: SiO₂ Substrates

Published on: March 7, 2014

Simple, Affordable, and Modular Patterning of Cells using DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

Main Results:

Micron-scale patterns of a poly(ethylene)-block-poly(ethylene oxide) copolymer, poly-L-tryptophan, and bovine collagen were successfully fabricated, mimicking the underlying hydrophilic patterns.
Polymer pattern height decreased with decreasing width for the block copolymer, attributed to structural instability.
Different molecular weights of poly-L-tryptophan exhibited distinct morphologies: smooth patterns for oligomers and fibrous structures for higher molecular weights.

Conclusions:

The developed microcontact printing method provides a versatile platform for creating complex polymer and biomaterial patterns.
The observed pattern morphology is influenced by polymer molecular weight and structural stability.
This technique holds potential for applications in biomaterials, microfluidics, and surface engineering.