Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wide-bore monolithic column for electrochromatography.

Ruijuan Yuan1, Guosheng Ding, Yugao Guo

  • 1School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P. R. China.

Electrophoresis
|May 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A real-world study of Faricimab: efficacy and biomarkers in treatment-experienced neovascular age-related macular degeneration(nAMD) and diabetic macular edema (DME) patients.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

A CREKA-modified multifunctional nanoplatform for targeted thrombolysis: a<i>in vitro</i>characterization of efficacy and biocompatibility for retinal thrombotic diseases.

Biomedical materials (Bristol, England)·2026
Same author

A multidimensional framework for dissociating the neuroplasticity of auditory and early language deprivation.

Communications biology·2026
Same author

Cluster-induced non-topological robust boundary states in a photonic lattice.

Optics letters·2026
Same author

Atypical Theta and Low Gamma Band Auditory Sampling in Chinese Children With Developmental Dyslexia: An ASSR Study.

Developmental science·2026
Same author

Anticoagulant peptide LR from Whitmania pigra ameliorates cerebral ischemia-reperfusion injury.

Brain research·2026

A novel wide-bore electrophoresis system with inner cooling was developed for electrochromatography. This setup enables high electric field strengths and large sample volumes, achieving good separation efficiency for test compounds.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Electrochromatography (EC) offers high separation efficiency.
  • Effective heat management is crucial for high-performance EC.

Purpose of the Study:

  • To develop and evaluate a novel wide-bore electrophoresis (WE) system with inner cooling for electrochromatography.
  • To assess the system's capability to handle high electric fields and large sample volumes.

Main Methods:

  • A wide-bore quartz tube (1.2 mm inner diameter) was utilized as the separation channel.
  • An inner cooling device with a coolant-circulating capillary was integrated for efficient heat dissipation.
  • A polyacrylamide monolithic column was prepared and evaluated in electrochromatographic mode.

Main Results:

Related Experiment Videos

  • The system successfully managed Joule heat, allowing for electric field strengths up to 625 V/cm.
  • The Ohm plot showed minimal current deviation, indicating stable operation.
  • The system accommodated sample volumes as large as 1 microL, yielding reasonable separation efficiency.

Conclusions:

  • The developed wide-bore electrophoresis system with inner cooling is effective for electrochromatography.
  • The system demonstrates potential for high-performance separations with enhanced thermal stability and sample capacity.