Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Guiding bacteria with small molecules and RNA.

Shana Topp1, Justin P Gallivan

  • 1Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.

Journal of the American Chemical Society
|May 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The next-generation CAR-T therapy landscape.

Nature reviews. Drug discovery·2023
Same author

Manufacturing innovation to drive down cell therapy costs.

Trends in biotechnology·2023
Same author

Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria.

Journal of bacteriology·2017
Same author

RNA synthetic biology: from the test tube to cells and back again.

ACS synthetic biology·2015
Same author

A family of synthetic riboswitches adopts a kinetic trapping mechanism.

Nucleic acids research·2014
Same author

Riboswitches for intracellular study of genes involved in Francisella pathogenesis.

mBio·2012

Scientists reprogrammed E. coli bacteria to follow new chemical signals using a synthetic riboswitch. This breakthrough enables precise bacterial localization for applications in bioremediation and synthetic biology.

Area of Science:

  • Microbiology
  • Synthetic Biology
  • Biotechnology

Background:

  • Bacteria navigate chemical environments using complex signaling pathways and molecular motors.
  • Reprogramming bacteria to sense novel molecules is challenging due to intricate signaling and protein engineering limitations.

Purpose of the Study:

  • To develop a method for reprogramming bacteria to follow entirely new chemical signals.
  • To demonstrate the use of synthetic riboswitches for novel ligand recognition in bacteria.

Main Methods:

  • Engineered E. coli with a synthetic riboswitch to detect a specific novel ligand.
  • Utilized the riboswitch system to guide bacterial chemotaxis towards the target chemical signal.

Main Results:

Related Experiment Videos

  • Successfully reprogrammed E. coli to exhibit chemotaxis towards a completely new chemical signal.
  • Achieved precise localization of bacteria to the novel chemical signal using the synthetic riboswitch system.

    • Conclusions:

    • Synthetic riboswitches offer a viable alternative to protein engineering for reprogramming bacterial chemotaxis.
    • This approach opens new avenues for bacteria in bioremediation, bionanotechnology, and synthetic biology applications.