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Related Experiment Videos

Quality control in antimicrobial disk susceptibility testing: a Belgian multicenter study.

S Lauwers1, J Philippe, A Van Zeebroeck

  • 1Department of Microbiology, Free University of Brussels, Belgium.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|August 1, 1991
PubMed
Summary
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Antimicrobial susceptibility testing quality control showed BBL disks had 10.4% errors, while Rosco tablets had 7.3%. Both systems demonstrated comparable precision, suggesting improved collaboration for optimal quality control limits.

Area of Science:

  • Medical Microbiology
  • Clinical Laboratory Science

Background:

  • Quality control (QC) is crucial for accurate antimicrobial susceptibility testing (AST).
  • Variability in QC results can impact patient treatment decisions.
  • Standardized methods are essential for reliable AST.

Purpose of the Study:

  • To evaluate quality control problems in antimicrobial disk susceptibility testing.
  • To compare the performance of BBL disks and Rosco tablets in AST QC.
  • To identify specific strain-antibiotic combinations causing major QC issues.

Main Methods:

  • A Belgian multicenter trial involving 18 laboratories over four weeks.
  • Daily testing of three reference strains against eight antibiotics.
  • Use of a standardized methodology for all participating laboratories.

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Main Results:

  • 10.4% of measurements with BBL disks were outside the recommended QC range.
  • 7.3% of measurements with Rosco tablets were outside the recommended QC range.
  • Comparable precision was observed between BBL disks and Rosco tablets.

Conclusions:

  • Specific strain-antibiotic combinations present significant challenges in AST QC.
  • Enhanced cooperation between manufacturers and reference laboratories is needed.
  • Establishing optimal quality control limits requires collaborative efforts.