Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tomographic Spectral Imaging with Multivariate Statistical Analysis: Comprehensive 3D Microanalysis.

Paul G Kotula1, Michael R Keenan, Joseph R Michael

  • 1Sandia National Laboratories, Materials Characterization Department, P.O. Box 5800, MS0886, Albuquerque, NM 87185-0886, USA. pgkotul@sandia.gov

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|May 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interlaboratory Comparison of Orbitrap Analyzers in OrbiSIMS: Noise Parameters, Intensity Scaling, and Detection Limits.

Journal of the American Society for Mass Spectrometry·2026
Same author

Realizing Cocktail Effects in Catalytic High-Entropy Metal-Organic Frameworks (HEMOFs) via Predictive Density of State Calculations.

Angewandte Chemie (International ed. in English)·2026
Same author

Orbitrap noise structure and method for noise unbiased multivariate analysis.

Nature communications·2025
Same author

High-Entropy Metal-Organic Frameworks (HEMOFs): A New Frontier in Materials Design for CO<sub>2</sub> Utilization.

Advanced materials (Deerfield Beach, Fla.)·2024
Same author

Hierarchical Self-Assembly of Carbon Dots into High-Aspect-Ratio Nanowires.

Nano letters·2023
Same author

Characterization of Anomalous Grains in FeCo Magnetic Alloys.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2023
Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Postnatal Developmental Expression and Localization of Water Channel Proteins in the Rat Ovary.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Novel Hybrid Semiconductor-Cellular Standard for 3D FIB-SEM Nanotomography Analysis.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Morpho-Anatomical Characterization of the Raisin Tree, Hovenia dulcis Thunb., Utilizing Optical Light and Scanning Electron Microscopy With Energy Dispersive X-Ray Spectroscopy.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Comparative Aspects of the Hemipenial Architecture of the Arabian Horned Viper Cerastes gasperettii: Macroscopic and Microscopic Analysis With Evolutionary Perspectives.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
See all related articles

Researchers developed a 3D microanalysis technique using scanning electron microscopy (SEM)/focused ion beam (FIB) and energy-dispersive X-ray spectroscopy (EDS). This tomographic spectral imaging (TSI) method enables detailed material analysis for applications like corrosion studies.

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Microscopy

Background:

  • Advanced materials characterization requires high-resolution 3D elemental mapping.
  • Existing techniques often lack the spatial resolution or elemental sensitivity for complex samples.

Purpose of the Study:

  • To develop a comprehensive 3D microanalysis procedure for detailed material investigation.
  • To demonstrate the capability of the developed technique for site-specific elemental analysis.

Main Methods:

  • Combined scanning electron microscope (SEM)/focused ion beam (FIB) system with energy-dispersive X-ray spectrometer (EDS).
  • Serial sectioning using FIB to remove material layer by layer.
  • Acquisition of X-ray spectral images at each section.
  • Concatenation of 2D spectral images into a 4D dataset (3D space + spectral information).

Related Experiment Videos

  • Analysis using automated X-ray spectral image analysis software.

    • Main Results:

    • Successfully generated 3D elemental maps from serial sectioning data.
    • Applied the technique to Cu-Ag eutectic and localized corrosion samples.
    • Demonstrated the site-specific analysis capability of tomographic spectral imaging (TSI).

    Conclusions:

    • The developed 3D microanalysis procedure provides comprehensive, site-specific elemental information.
    • Tomographic spectral imaging (TSI) combined with multivariate statistical analysis is powerful for complex material studies.
    • This technique advances the understanding of material microstructures and degradation mechanisms.