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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Related Experiment Video

Updated: Jul 15, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Imaging atomic structure in metal nanoparticles using high-resolution cryo-TEM.

Olivier Balmes1, Jan-Olle Malm, Niklas Pettersson

  • 1National Center for High Resolution Electron Microscopy, Department of Materials Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|May 8, 2007
PubMed
Summary

Achieving sub-0.20-nm resolution in cryo-transmission electron microscopy (cryo-TEM) is possible with standard equipment. This breakthrough enables detailed imaging of atomic structures in materials like gold nanoparticles.

More Related Videos

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Related Experiment Videos

Last Updated: Jul 15, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Area of Science:

  • Materials Science
  • Microscopy
  • Nanotechnology

Background:

  • High-resolution imaging is crucial for understanding material properties at the nanoscale.
  • Cryo-transmission electron microscopy (cryo-TEM) offers a method for studying materials in a near-native state.

Purpose of the Study:

  • To demonstrate sub-0.20-nm structural resolution in cryo-TEM.
  • To validate imaging capabilities for nanoscale materials.

Main Methods:

  • Utilized a 300-kV field emission gun (FEG) TEM with commercially available cryo equipment.
  • Imaged 15-nm gold particles embedded in amorphous frozen water.
  • Employed Fourier transform analysis to confirm resolution.
  • Supported experimental findings with multislice image simulations.

Main Results:

  • Achieved better than 0.20-nm structural resolution, visualizing gold (111) planes (0.235 nm).
  • Demonstrated the presence of gold (200) planes in images, confirming the resolution.
  • Image simulations indicated potential for similar resolution in smaller and lighter element particles.

Conclusions:

  • Sub-0.20-nm resolution is attainable in cryo-TEM using standard equipment.
  • Further research should focus on minimizing amorphous film thickness and electron dose.
  • This resolution level opens new possibilities for nanoscale material characterization.