Related Experiment Video
Updated: Jul 15, 2026
07:59
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
Published on: August 19, 2021
LC/MS quantitative study of glucose by iodine attachment
Eduard Rogatsky1, Vlad Tomuta, Daniel T Stein
1Analytical Core Laboratory, General Clinical Research Center, Albert Einstein College of Medicine of Yeshiva University, United States. rogatsky@aecom.yu.edu
Analytica Chimica Acta
|May 8, 2007
Summary
Iodine attachment enhances glucose measurement sensitivity using liquid chromatography-mass spectrometry (LC/MS). This method simplifies sample preparation and boosts throughput, offering a superior alternative to GC/MS for accurate glucose quantification.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Accurate glucose measurement is crucial for diagnosing and managing diabetes.
- Existing methods like Gas Chromatography-Mass Spectrometry (GC/MS) have limitations in sensitivity and sample preparation complexity.
Purpose of the Study:
- To investigate iodine attachment as a novel approach to enhance glucose detection sensitivity in LC/MS.
- To develop and optimize a method for glucose analysis using iodine attachment and LC/MS.
Main Methods:
- Glucose samples were prepared and separated using normal phase chromatography.
- Anionization was achieved via iodine (I(-)) attachment using post-column addition of iodoform.
- Quantification of glucose and deuterated glucose ([6,6-(2)H2]glucose) was performed using negative ion mode electrospray mass spectrometry in selected ion monitoring (SIM) mode.
Main Results:
- Iodine attachment LC/MS demonstrated high sensitivity, surpassing GC/MS.
- The method significantly simplified sample preparation and increased analytical throughput.
- A limit of detection (LOD) of 50 pg glucose on column was achieved.
- A gradient-based delivery platform was developed to optimize iodoform concentration, addressing sublimation challenges.
Conclusions:
- Iodine attachment is a highly effective strategy for improving glucose sensitivity in LC/MS analysis.
- This technique offers advantages in simplicity, throughput, and sensitivity, applicable even with older mass spectrometry instruments.
- Method development strategies for optimizing iodoform concentration are provided.
