An amperometric glucose biosensor constructed by immobilizing glucose oxidase on titanium-containing mesoporous...

We have constructed a glucose biosensor by immobilizing glucose oxidase (GOD) on titanium-containing MCM-41 (Ti-MCM-41) modified screen-printed electrodes. The strategy of the sensing method is to monitor the extent of the decrease of the reduction current of O2 upon adding glucose at a selected potential. The detection can be done at the applied potential of -0.50 V and can efficiently exclude the interference from commonly coexisted substances. The constructed sensor has a high sensitivity to glucose (5.4 mA M(-1) cm(-2)) and a linear response range of 0.10-10.0 mM. The detection limit is 0.04 mM at a signal-to-noise ratio of 3. The sensor also shows high stability and remains its catalytic activity up to 60 degrees C. The biocompatibility of Ti-MCM-41 means that this immobilization matrix not only can be used for immobilizing GOD but also can be extended to other enzymes and bioactive molecules, thus providing a promising platform for the development of biosensors.