A new rapid fluorogenic method for measuring bacteriocin activity
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Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Published on: June 12, 2013
Norma de la Fuente-Salcido1, Rubén Salcedo-Hernández, Ma Guadalupe Alanís-Guzmán
1Universidad de Guanajuato, Departamento de Ingeniería en Alimentos, Instituto de Ciencias Agrícolas, Irapuato, Guanajuato, 36500 Mexico.
Journal of Microbiological Methods
|May 8, 2007
Abstract:
We describe a novel bacteriocin screening assay based on fluorescence emitted by berberine following its influx into compromised cells. This technique showed agreement with the conventional well-diffusion method, and results can be obtained within one hour. This assay could facilitate the rapid identification of bacteriocinogenic bacterial isolates.
