Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Determining membrane protein structures: still a challenge!

Jean-Jacques Lacapère1, Eva Pebay-Peyroula, Jean-Michel Neumann

  • 1INSERM, U773, Centre de Recherche Biomédicale Bichat Beaujon CRB3, Faculté de Médecine X. Bichat, Université Paris 7, BP 416, F-75018, Paris, France. lacapere@bichat.inserm.fr

Trends in Biochemical Sciences
|May 8, 2007
PubMed
Summary

Determining transmembrane protein structures and dynamics is crucial for understanding their function. Advanced methods are needed to overcome challenges in obtaining high-resolution 3D structures for drug design.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MtGRF8 interacts with MtSymCRK and prevents early bacteroid death during Medicago - Sinorhizobium symbiosis.

The New phytologist·2026
Same author

Conformational dynamics study of TfR1 upon transferrin binding via NMA and MD simulations.

Journal of molecular graphics & modelling·2026
Same author

ANABAG: Annotated Antibody-Antigen Data Set with Unique Features for Antibody Engineering Applications.

Journal of chemical information and modeling·2025
Same author

The key role of the dynamics and flexibility of proteins in functional mechanisms: How computational methods can contribute to their identification.

Biochimie·2025
Same author

TSPO, reactive oxygen species and oxidative stress in physiological and pathological situations: a complex relationship.

Biochimie·2025
Same author

Cancer-associated loss-of-function mutations in KCNQ1 enhance Wnt/β-catenin signalling disrupting epithelial homeostasis.

Oncogene·2025

Area of Science:

  • Structural biology
  • Biochemistry
  • Molecular dynamics

Background:

  • Transmembrane proteins are vital for cellular functions.
  • Understanding their structure and dynamics is key to elucidating function.
  • Obtaining high-resolution 3D structures of these proteins is challenging.

Purpose of the Study:

  • To highlight the importance of determining transmembrane protein structures and dynamics.
  • To discuss the challenges in obtaining atomic-level 3D structures.
  • To emphasize the need for integrated approaches combining structural and dynamic analyses.

Main Methods:

  • X-ray diffraction
  • Electron microscopy
  • Nuclear Magnetic Resonance (NMR) spectroscopy

Related Experiment Videos

  • Computational modeling

    • Main Results:

    • High-resolution 3D structures and molecular dynamics analyses are required for understanding protein function.
    • Purification of functional transmembrane proteins presents a significant bottleneck.
    • A combination of structural biology techniques is often necessary.

    Conclusions:

    • Progress in determining transmembrane protein structures will enhance understanding of ligand-induced activation/inhibition.
    • Improved structural insights will aid in the design of targeted drugs for membrane proteins.
    • Integrated structural and dynamic studies are essential for advancing membrane protein research.