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Related Experiment Videos

Adequate ovarian follicular status does not prevent the decrease in pregnancy rates associated with high sperm DNA

Nelly Frydman1, Nadia Prisant, Laetitia Hesters

  • 1Department of Genetics and Reproduction, Antoine Béclère Hospital, Clamart, France. nelly.frydman@abc.ap-hop-paris.fr

Fertility and Sterility
|May 8, 2007
PubMed
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High sperm DNA fragmentation negatively impacts in vitro fertilization success rates, despite not affecting fertilization or embryo quality. This finding is crucial for understanding male infertility factors in assisted reproduction.

Area of Science:

  • Reproductive Medicine
  • Sperm Biology
  • In Vitro Fertilization

Background:

  • Sperm DNA fragmentation (SDF) is a potential indicator of male infertility.
  • The impact of SDF on IVF-ET outcomes is debated, partly due to potential oocyte repair mechanisms.
  • This study aimed to isolate the effect of SDF on IVF-ET outcomes in women with optimal ovarian function.

Purpose of the Study:

  • To investigate the association between high sperm DNA fragmentation and IVF-ET success rates.
  • To determine if SDF affects fertilization, embryo quality, implantation, or pregnancy rates.
  • To exclude the confounding factor of oocyte repair by using women with healthy ovarian function.

Main Methods:

  • Prospective study conducted at a teaching hospital in France.

Related Experiment Videos

  • 117 women under 38 with normal ovarian reserve markers underwent conventional IVF-ET.
  • Sperm DNA fragmentation was assessed using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and categorized as high (HF, >35%) or low (LF, ≤35%).

    • Main Results:

    • No significant differences in fertilization rates or D2 embryo quality were observed between HF and LF groups.
    • Implantation rates were significantly lower in the HF group (24.5%) compared to the LF group (42.4%).
    • Clinical pregnancy rates (37.5% vs. 62.5%) and ongoing pregnancy rates (23.5% vs. 57.8%) were substantially reduced in the HF group.

    Conclusions:

    • High sperm DNA fragmentation does not impair fertilization or early embryo morphology.
    • SDF is significantly associated with reduced implantation and ongoing pregnancy rates following IVF-ET.
    • SDF is a critical factor negatively impacting IVF-ET outcomes, even in women with excellent ovarian function.