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Updated: Jul 15, 2026
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia
Published on: August 1, 2025
NGF-induced reduction of an outward-rectifying TRPM7-like current in rat CA1 hippocampal neurons
Shun-Lian Tian1, Hui Jiang, Yan Zeng
1Department of Neurobiology and Key Laboratory of Neurological Disease of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
Abstract:
Transient receptor potential melastatin 7 (TRPM7) channels are widely expressed in the nervous system; however, their function and regulation are largely unknown. This study aimed to explore whether the current mediated by TRPM7-like channels in rat CA1 hippocampal neurons could be modulated by nerve growth factor (NGF). Using whole-cell patch clamp techniques, we identified an outward-rectifying TRPM7-like current in hippocampal neurons freshly isolated from postnatal (10-day-old) rats. The outward component of this current was reversibly reduced by NGF in dose- and time-dependent manners, and this effect was substantially blocked by K252a, an inhibitor of TrkA. In addition, NGF-induced reduction of the TRPM7-like current was abolished by U73122, a phopholipase C inhibitor. In light of the abundance of NGF in hippocampus that express both TrkA and TRPM7, these results suggest that the function of TRPM7-like channels in hippocampal neurons may be regulated by NGF.
