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[In vitro transmission of mouse leukemia virus to restrictive mouse cells: necessity for mixed infection by two
Abstract:
The plaque formation of murine leukemia virus (MuLV) in non permissive mouse cells (N-tropic MuLV in B-type cells, or B-tropic MuLV in N-type cells) was increased by murine sarcoma virus whose plaque-forming activity was extremely low (MuSV XC-). The infection of N-tropic MuLV in B-type cells was increased by MuSV XC- propagated only in N-type cells but not in B-type cells, and the infection of B-tropic MuLV in N-type cells by MuSV XC- propagated only in B-type cells but not in N-type cells.
Insights
Murine sarcoma virus (MuSV XC-) enhances plaque formation of murine leukemia virus (MuLV) in specific mouse cell types. This enhancement depends on how MuSV XC- is propagated, showing cell-type specific interactions.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Context:
- Murine leukemia virus (MuLV) plaque formation is crucial for studying viral replication and infectivity in host cells.
- Non-permissive cell lines, such as N-type and B-type mouse cells, typically restrict MuLV infection.
- Murine sarcoma virus (MuSV XC-) with low plaque-forming activity was investigated for its effect on MuLV infection.
Purpose:
- To investigate the influence of a low-plaque-forming murine sarcoma virus (MuSV XC-) on the plaque formation of murine leukemia virus (MuLV).
- To determine the cell-type specificity of MuSV XC- in enhancing MuLV infection in non-permissive mouse cells.
Summary:
- Murine sarcoma virus (MuSV XC-) significantly increased plaque formation of murine leukemia virus (MuLV) in non-permissive mouse cells.
- N-tropic MuLV infection in B-type cells was enhanced by MuSV XC- propagated in N-type cells, but not when propagated in B-type cells.
- B-tropic MuLV infection in N-type cells was enhanced by MuSV XC- propagated in B-type cells, but not when propagated in N-type cells.
Impact:
- This study reveals a cell-type dependent interaction between MuSV XC- and MuLV, influencing viral infectivity.
- The findings highlight the importance of viral propagation conditions in determining the outcome of co-infections.
- Understanding these interactions can provide insights into viral tropism and host-pathogen relationships.