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legless insertional mutation: morphological, molecular, and genetic characterization.

G Singh1, D M Supp, C Schreiner

  • 1Children's Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Ohio 45229.

Genes & Development
|December 1, 1991
PubMed
Summary
This summary is machine-generated.

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The legless (lgl) mutation in mice causes severe limb, brain, and craniofacial defects, alongside situs inversus. This study identifies the mutation

Area of Science:

  • Developmental Biology
  • Genetics
  • Teratology

Background:

  • Limb morphogenesis serves as a key model for vertebrate pattern formation.
  • The legless (lgl) mutation offers a tool to study limb development genetically and molecularly.

Purpose of the Study:

  • To investigate the developmental, genetic, and molecular basis of the legless (lgl) mutation.
  • To characterize the limb malformations and identify the affected genes.

Main Methods:

  • Morphological analysis of mutant hindlimb buds.
  • Complementation tests with the situs inversus (iv) mutation.
  • Molecular analysis including cloning and mapping of transgene insertion sites.
  • Isolation of candidate cDNAs for lgl and iv.

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Main Results:

  • Homozygous legless mutants display severe limb, brain, and craniofacial malformations, and situs inversus.
  • Absence of the apical ectodermal ridge and increased cell death were observed in mutant hindlimb buds.
  • Mutants showed extreme sensitivity to retinoic acid.
  • Complementation tests confirmed allelism between lgl and iv, suggesting disruption of multiple genes.
  • Molecular mapping placed the insertions near the iv locus on chromosome 12.

Conclusions:

  • The legless mutation disrupts genes essential for limb development, craniofacial formation, and visceral patterning.
  • The mutation's effects are influenced by genetic background, indicating interaction with other genes.
  • The legless mutation is allelic to situs inversus viscerum (iv), potentially affecting multiple genes at the insertion site.