Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reduced c-myb expression levels affect hematopoietic development in vitro.

Chen Jieping1, Deborah Clarke, Constanze Bonifer

  • 1Division of Experimental Haematology, Leeds Institute of Molecular Medicine, St. James's University Hospital, University of Leeds, Leeds, UK. chenjpxn@yahoo.com.cn

International Journal of Hematology
|May 8, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic insights into gene regulation driving normal and malignant hematopoietic development from embryonic and induced pluripotent stem cell differentiation.

Experimental hematology·2026
Same author

Impact of Sex on Prediction of Asthma Attacks by Clinical Risk Factors and Type 2 Biomarkers.

Chest·2026
Same author

Effectiveness of biologics for reducing occlusive mucus plugs in patients with severe asthma: a systematic review.

Respiratory research·2026
Same author

Effect of tezepelumab on asthma exacerbations co-occurring with infection-attributed acute respiratory illnesses.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2025
Same author

Fusion gene depletion eliminates stemness and induces bidirectional differentiation of acute myeloid leukemia.

Blood·2025
Same author

A heterozygous CEBPA mutation disrupting the bZIP domain in a RUNX1 and SRSF2 mutational background causes MDS disease progression.

Nature communications·2025

Reduced c-myb expression significantly alters hematopoietic progenitor development, particularly erythroid differentiation, in embryonic stem cells. Even a slight decrease impacts developmental kinetics.

Area of Science:

  • Hematopoiesis
  • Stem Cell Biology
  • Molecular Biology

Background:

  • c-myb is a crucial transcription factor for early hematopoietic development.
  • Understanding c-myb's role is key to deciphering hematopoietic differentiation pathways.

Purpose of the Study:

  • To investigate the impact of reduced c-myb expression on hematopoietic development kinetics.
  • To determine if c-myb levels affect progenitor cell development in differentiating embryonic stem cells.

Main Methods:

  • Induced hematopoietic differentiation of embryonic stem cells in methylcellulose without LIF.
  • Analyzed hematopoietic cells using methylcellulose colony assay.
  • Examined gene expression patterns via real-time polymerase chain reaction.

Related Experiment Videos

Main Results:

  • A reduction in c-myb expression to 55% significantly altered progenitor development kinetics.
  • Erythroid differentiation was notably affected by decreased c-myb levels.
  • No significant changes were observed in the expression of specific erythroid marker genes.

Conclusions:

  • c-myb plays a critical role in regulating the kinetics of hematopoietic progenitor development.
  • Erythroid differentiation is particularly sensitive to c-myb dosage.
  • Further research is needed to understand the discrepancy between altered differentiation and unchanged marker gene expression.