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Related Experiment Video

Updated: Jul 15, 2026

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

Self-formed adaptor PCR: a simple and efficient method for chromosome walking.

Shiming Wang1, Jian He, Zhongli Cui

  • 1Department of Microbiology, Nanjing Agricultural University, No 1 Weigang, Nanjing, PR China.

Applied and Environmental Microbiology
|May 8, 2007
PubMed
Summary

We developed Self-Formed Adaptor PCR (SEFA PCR) for efficient chromosome walking. This method amplifies long flanking sequences, aiding in the isolation of unknown DNA segments in complex genomes.

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Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • Chromosome walking is crucial for genome sequencing and analysis.
  • Existing methods for isolating unknown flanking sequences can be complex and inefficient.

Purpose of the Study:

  • To develop a novel, efficient method for chromosome walking.
  • To facilitate the isolation of unknown DNA sequences from complex genomes.

Main Methods:

  • Development of Self-Formed Adaptor Polymerase Chain Reaction (SEFA PCR).
  • Application of SEFA PCR for amplifying flanking sequences.

Main Results:

  • SEFA PCR successfully amplified flanking sequences.
  • Most amplified sequences exceeded 2.0 kb, with some reaching 6.0 kb.

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Associated Chromosome Trap for Identifying Long-range DNA Interactions
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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

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Last Updated: Jul 15, 2026

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

  • The method proved to be simple and efficient.

    • Conclusions:

    • SEFA PCR is a valuable tool for chromosome walking.
    • The technique offers broad applications for isolating unknown sequences in complex genomes.