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Updated: Jul 15, 2026
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Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
Can we talk about CD4+CD28- lymphocytes as a risk factor for ischemic stroke?
M Nowik1, P Nowacki, J Grabarek
1Department of Neurology, Pomeranian Medical University, Szczecin, Poland. nowiczontko@poczta.onet.pl
European Neurology
|May 8, 2007
Summary
CD4+CD28- lymphocytes are linked to increased risk of ischemic stroke. These immune cells, implicated in atherosclerosis, were found in higher percentages in stroke patients compared to healthy individuals.
Area of Science:
- Immunology
- Neurology
- Cardiovascular Science
Background:
- CD4+CD28- lymphocytes are associated with atheromatous plaque destabilization and acute coronary events.
- Their potential role in ischemic stroke pathogenesis, particularly in patients with atherosclerosis, requires investigation.
Purpose of the Study:
- To investigate the association between CD4+CD28- lymphocytes and ischemic stroke.
- To compare the prevalence of these lymphocytes in acute ischemic stroke patients, patients with atherosclerosis risk factors, and healthy controls.
Main Methods:
- Flow cytometry was used to quantify CD4+CD28- lymphocyte percentages in peripheral blood.
- Patients were categorized into acute ischemic stroke, high-risk atherosclerosis, and healthy control groups.
Main Results:
- CD4+CD28- lymphocyte percentages were significantly higher in both ischemic stroke and high-risk atherosclerosis groups compared to controls.
- No significant difference was observed between the ischemic stroke and high-risk atherosclerosis groups.
- No correlation was found between lymphocyte levels and stroke severity, CT findings, or time since stroke onset.
Conclusions:
- CD4+CD28- lymphocytes are implicated in the mechanisms that increase the risk of acute ischemic stroke.
- These cells are a contributing factor to stroke risk, rather than a consequence of the stroke event itself.
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