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Updated: Jul 15, 2026
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Published on: January 3, 2014
Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato-virus-X-based binary
Jingying Liu1, Pengda Ma, Yan Sun
1Institute of Genetics and Cytology, Northeast Normal University, 5268 Renmin Street, Changchun 130024, People's Republic of China.
Abstract:
The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.
