Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fine Adjustment of Caenorhabditis elegans Orientation on Channeled Agar Pads for Imaging Neuroregeneration05:12

Fine Adjustment of Caenorhabditis elegans Orientation on Channeled Agar Pads for Imaging Neuroregeneration

743
Here, we present a protocol for fabricating channeled agar pads using PDMS molds created from vinyl records. The channels enable users to finely orient Caenorhabditis elegans to improve imaging contrast and facilitate the comparison of structures. These capabilities are particularly useful in neuroregeneration studies.
743
Correction of Presbyopia by Monocular Bi-Aspheric Ablation Profile05:46

Correction of Presbyopia by Monocular Bi-Aspheric Ablation Profile

726
This article provides a step-by-step guide to correcting presbyopia with a monocular bi-aspheric ablation...
726
An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

14.7K
This protocol is a beginner's entryway into processing, fitting, and interpreting transient absorption spectra. The focus of this protocol is the preparation of datasets, and fitting using both single wavelength kinetics and global lifetime analysis. Challenges associated with transient absorption data and its fitting are discussed.
14.7K
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon08:18

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

7.9K
Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software,...
7.9K
Operation of the Collaborative Composite Manufacturing (CCM) System10:09

Operation of the Collaborative Composite Manufacturing (CCM) System

7.0K
A collaborative composite manufacturing system is developed for robotic lay-up of composite laminates using the prepreg tape. The proposed system allows the production of composite laminates with high levels of geometrical complexity. The issues in the path planning, coordination of the robots and control are addressed in the proposed...
7.0K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

10.1K
A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.
10.1K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Hepatitis B Virus X Protein Induces Hepatic Steatosis Via Transcriptional Activation Of Srebp1 And Ppargamma.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Hepatitis B Virus X Protein Induces Hepatic Steatosis Via Transcriptional Activation Of Srebp1 And Ppargamma.

Related Experiment Video

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity
05:46

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity

Published on: September 20, 2024

726

Hepatitis B virus X protein induces hepatic steatosis via transcriptional activation of SREBP1 and PPARgamma.

Kook Hwan Kim1, Hye-Jun Shin, Kyeongjin Kim

  • 1Department of Molecular Biology, Pusan National University, Busan, Korea.

Gastroenterology
|May 9, 2007

View abstract on PubMed

Summary
This summary is machine-generated.

Hepatitis B virus X protein (HBx) induces liver fat accumulation by increasing sterol regulatory element binding protein 1 and PPARgamma. This provides a molecular mechanism for hepatitis B virus-related liver disease.

More Related Videos

Operation of the Collaborative Composite Manufacturing CCM System
10:09

Operation of the Collaborative Composite Manufacturing CCM System

Published on: October 1, 2019

7.0K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

10.1K

Related Experiment Videos

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity
05:46

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity

Published on: September 20, 2024

726
Operation of the Collaborative Composite Manufacturing CCM System
10:09

Operation of the Collaborative Composite Manufacturing CCM System

Published on: October 1, 2019

7.0K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

10.1K

Area of Science:

  • Hepatology
  • Molecular Biology
  • Virology

Background:

  • Hepatic steatosis is common in chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infections.
  • Steatosis acts as a cofactor in liver fibrosis, hepatitis, and cancer.
  • The molecular mechanism of HBV-induced steatosis remains unclear.

Purpose of the Study:

  • To elucidate the molecular mechanism by which hepatitis B virus X protein (HBx) induces hepatic steatosis.
  • To investigate the role of HBx in lipid metabolism in liver cells.

Main Methods:

  • Investigated lipid accumulation and gene expression in HBx-transfected liver cells and HBx-transgenic mice.
  • Analyzed the expression of sterol regulatory element binding protein 1 (SREBP1) and peroxisome proliferator-activated receptor gamma (PPARγ).

Main Results:

  • HBx overexpression led to hepatic lipid accumulation in cells and mice.
  • HBx up-regulated SREBP1 and PPARγ expression and activity.
  • HBx increased the expression of lipogenic and adipogenic enzymes.

Conclusions:

  • Increased HBx expression causes hepatic lipid accumulation via SREBP1 and PPARγ.
  • This pathway represents a potential molecular mechanism in HBV pathophysiology.
  • HBx-induced steatosis may contribute to liver disease progression in HBV infection.