Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

Overview of Hematopoiesis

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Production of Formed Elements

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stromal STAT5-Mediated Trophic Activity Regulates Hematopoietic Niche Factors.

Stem cells (Dayton, Ohio)·2023

Same author

JMML tumor cells disrupt normal hematopoietic stem cells by imposing inflammatory stress through overproduction of IL-1β.

Blood advances·2021

Same author

Pimozide and Imipramine Blue Exploit Mitochondrial Vulnerabilities and Reactive Oxygen Species to Cooperatively Target High Risk Acute Myeloid Leukemia.

Antioxidants (Basel, Switzerland)·2021

Same author

Gab2 and Gab3 Redundantly Suppress Colitis by Modulating Macrophage and CD8<sup>+</sup> T-Cell Activation.

Frontiers in immunology·2019

Same author

The Cooperative Relationship between STAT5 and Reactive Oxygen Species in Leukemia: Mechanism and Therapeutic Potential.

Cancers·2018

Same author

Global loss of leucine carboxyl methyltransferase-1 causes severe defects in fetal liver hematopoiesis.

The Journal of biological chemistry·2018

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

STAT5 signaling in normal and pathologic hematopoiesis.

Kevin D Bunting¹

¹Department of Medicine, Division of Hematology/Oncology, Case Western Reserve University School of Medicine, Cleveland, OH 44122-7284, USA. Kevin.Bunting@case.edu

Frontiers in Bioscience : a Journal and Virtual Library

|May 9, 2007

Summary

Signal transducer and activator of transcription 5 (STAT5) activation is crucial for normal hematopoietic stem cell development and is implicated in leukemogenesis. Understanding STAT5

More Related Videos

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Published on: November 3, 2017

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

Related Experiment Videos

Last Updated: Jul 15, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Published on: November 3, 2017

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

Area of Science:

Hematology
Molecular Biology
Oncology

Background:

Hematopoietic development relies heavily on cytokine/receptor signaling pathways.
The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is central to hematopoietic cell function.
STAT5 activation plays a critical role in both normal and malignant hematopoiesis.

Purpose of the Study:

To review the multifaceted role of STAT5 activation in hematopoietic stem cells and progenitor cells.
To explore the association of STAT5 activation with hematologic malignancies and cytokine-independent cell expansion.
To highlight the significance of STAT5 in the development of targeted therapeutics for hematologic cancers.

Main Methods:

Literature review focusing on STAT5 signaling in hematopoiesis.
Analysis of STAT5 activation mechanisms by cytokines, receptor tyrosine kinases, and JAK kinases.
Examination of STAT5 interactions with other signaling pathways, such as PI3K via Gab2.

Main Results:

STAT5 is essential for robust repopulation and proliferation in normal hematopoietic stem cells.
Constitutive STAT5 activation is linked to hematologic malignancies, promoting cell expansion.
STAT5 cooperates with pathways like PI3K, influencing leukemogenesis.

Conclusions:

STAT5 is a key player in normal hematopoietic stem cell function and aberrant in leukemogenesis.
Further research into STAT5 structure-function relationships is needed to distinguish normal from leukemic activation.
Targeting STAT5 pathways offers a promising therapeutic strategy for hematologic malignancies.