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Published on: January 7, 2020
Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome
Chelsee Ann Hewitt1, Catherine Louise Carmichael, Ella Jane Wilkins
1The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia. chewitt@wehi.edu.au
Abstract:
The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
