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Related Concept Videos

Candidiasis01:20

Candidiasis

Candidiasis is a fungal infection caused by opportunistic species of Candida. It can affect various anatomical sites, including the skin, oral cavity, nails, and genitourinary tract. Among its forms, vaginal candidiasis is the most common type of mucosal infection. It typically results from the overgrowth of Candida albicans in the vaginal mucosa. Under normal conditions, C. albicans exists as a commensal organism within the vaginal microbiota, regulated by the dominance of lactobacilli, which...
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Antifungal Agents01:15

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...
Trichomoniasis01:18

Trichomoniasis

Trichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, one of the most prevalent non-viral sexually transmitted infections in the United States. This extracellular parasite primarily colonizes the lower genitourinary tract in women—particularly the vagina—and in men, the urethra and prostate. Its structural and functional adaptations enable its survival, motility, and pathogenicity within the host environment.Structural Features and Host EntryT.

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Related Experiment Video

Updated: Jul 15, 2026

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
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A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

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Adherence and blocking of Candida albicans to cultured vaginal epithelial cells: treatments to decrease adherence.

Cara Hollmer1, Michael Essmann, Kevin Ault

  • 1Office of University Research, Des Moines University, Des Moines, IA 50312, USA.

Infectious Diseases in Obstetrics and Gynecology
|May 9, 2007
PubMed
Summary

Certain vaginal lubricants can reduce Candida yeast adherence to vaginal epithelial cells (VEC). This study used flow cytometry to demonstrate how products affect fungal adhesion, offering insights into managing mucosal infections.

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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Published on: December 8, 2011

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Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
07:57

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Published on: December 8, 2011

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Microbial pathogenesis relies on adherence to host tissues.
  • Candida albicans adherence to mucosal tissues may be influenced by cell surface hydrophobicity.

Purpose of the Study:

  • To evaluate the adherence of Candida albicans to vaginal epithelial cells (VEC).
  • To assess the inhibitory effect of commercial products on yeast adherence to VEC.

Main Methods:

  • Cultured a continuous cell line of vaginal epithelial cells (VEC).
  • Utilized fluorescein isothiocyanate-stained yeast cells for adherence assays.
  • Employed flow cytometry to quantify yeast cell binding to VEC.

Main Results:

  • Candida cells effectively bound to cultured VEC.
  • All tested K-Y lubricant formulations reduced Candida binding to VEC by 25% to 50%.
  • Overgrown or stressed VEC exhibited increased yeast cell binding.

Conclusions:

  • Flow cytometry is effective for assessing yeast cell adherence to cultured VEC.
  • Commercial products can interfere with yeast adherence to epithelial cells, suggesting potential for managing mucosal infections.