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Updated: Jul 15, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
[Characteristics of Vibrio parahaemolyticus isolated from mussels (Perna perna) commercialized at Niterói, Rio de
Christiane Soares Pereira1, Cristina de Albuquerque Possas, Célio Mauro Viana
1Laboratório de Enterobactérias, Departamento de Bacteriologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ. chrisspm@ioc.fiocruz.br
Abstract:
The marine ecosystem is the natural habitat of bacteria like Vibrio parahaemolyticus, an important pathogen that cause human gastroenteritis associated with seafood consumption. In the present investigation, the presence of V. parahaemolyticus in 86 in natural and precooked mussel samples was evaluated. Vibrio parahaemolyticus was isolated from 11.6% of the in natural and precooked mussels. All strains tested were urease-positive and 28.5% were Kanagawa-positive, which suggests that they have pathogenic potential for humans. There was predominance of the O10:K52 serotype and the emerging O3:K6 strain was identified. These results show the epidemiological relevance of V. parahaemolyticus in cases of human gastroenteritis following mussel consumption without adequately cooking them (100 degrees C/15 min). Moreover, it is important to alert the Brazilian Sanitary Surveillance authorities regarding their presence in the food chain and their public health risks.
Insights
Vibrio parahaemolyticus bacteria were found in 11.6% of mussel samples. These bacteria, some pathogenic, pose a public health risk through seafood consumption, especially if mussels are undercooked.
Area of Science:
- Marine microbiology
- Food safety
- Bacteriology
Background:
- Vibrio parahaemolyticus is a marine bacterium causing human gastroenteritis.
- Seafood consumption is a common route for Vibrio parahaemolyticus infection.
- Mussels are a potential vehicle for this pathogen.
Purpose of the Study:
- To determine the prevalence of Vibrio parahaemolyticus in mussel samples.
- To assess the pathogenic potential of isolated strains.
- To inform public health and sanitary surveillance.
Main Methods:
- Isolation and identification of Vibrio parahaemolyticus from mussel samples.
- Urease and Kanagawa tests to assess pathogenicity.
- Serotyping to identify specific strains.
Main Results:
- Vibrio parahaemolyticus was detected in 11.6% of natural and precooked mussel samples.
- All isolated strains were urease-positive; 28.5% were Kanagawa-positive, indicating pathogenic potential.
- O10:K52 was the predominant serotype, with the emerging O3:K6 strain also identified.
Conclusions:
- Vibrio parahaemolyticus is epidemiologically relevant in mussel consumption.
- Undercooked mussels pose a risk of gastroenteritis due to this pathogen.
- Alerts are necessary for Brazilian Sanitary Surveillance regarding food chain contamination and public health risks.
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