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Updated: Jul 15, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Diagnostic approach to hemoglobinopathies.

Ferdane Kutlar¹

¹Titus H.J. Huisman Hemoglobinopathy Laboratory, Sickle Cell Center, Medical College of Georgia, Augusta, Georgia, USA. fkutlar@mail.mcg.edu

|May 9, 2007

Summary

Diagnosing hemoglobinopathies, common inherited blood disorders, involves a stepwise approach. This includes clinical history, blood tests, protein analysis, and genetic methods for accurate identification.

Area of Science:

Hematology
Medical Genetics

Background:

Hemoglobinopathies are common inherited disorders affecting hemoglobin synthesis.
These disorders are classified as quantitative (thalassemias) or qualitative (variant Hbs).

Purpose of the Study:

To present a stepwise algorithmic approach for the definite identification of hemoglobinopathies.
To review the application of diagnostic methods at a specialized Sickle Cell Center Laboratory.

Main Methods:

Clinical history and hematologic evaluation (CBC, reticulocyte count, RBC morphology).
Protein-based methods: Hb electrophoresis, isoelectric focusing (IEF), cation exchange and reversed-phase HPLC.
Nucleic acid-based methods: PCR, RT-PCR, DNA/cDNA sequencing.
Functional/stability tests: p50 determination, Heinz body preparation, isopropanol/heat stability tests.

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Last Updated: Jul 15, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

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Main Results:

A comprehensive diagnostic algorithm integrates various analytical techniques.
The described methods enable precise identification of both quantitative and qualitative hemoglobin abnormalities.
Confirmatory tests and family studies aid in diagnosing functional or stability issues.

Conclusions:

A systematic, multi-method approach is crucial for accurate hemoglobinopathy diagnosis.
The integration of hematologic, protein-based, and nucleic acid-based methods ensures comprehensive evaluation.
Specialized laboratory reviews demonstrate the practical application of these diagnostic strategies.