Related Experiment Video
Updated: Jul 15, 2026
07:25
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
Published on: June 25, 2020
Abstract:
APCR and monozoospore plating was used to demonstrate a simultaneous presence of the mitochondria with mitochondrial DNA of haplotypes ILa and IIa in the mycelium of several Phytophthora infestans strains.
Insights
Mitochondria and their DNA, specifically haplotypes ILa and IIa, were found together in Phytophthora infestans mycelium. This simultaneous presence was confirmed using APCR and monozoospore plating techniques.
Area of Science:
- Molecular Biology
- Mycology
- Genetics
Context:
- Phytophthora infestans is a significant plant pathogen.
- Understanding its genetic makeup is crucial for disease management.
- Mitochondrial DNA (mtDNA) plays a key role in cellular functions and inheritance.
Purpose:
- To investigate the co-occurrence of different mitochondrial DNA haplotypes within Phytophthora infestans strains.
- To utilize advanced molecular techniques like APCR and monozoospore plating for accurate detection.
Summary:
- The study successfully demonstrated the simultaneous presence of mitochondria containing both haplotype ILa and IIa mitochondrial DNA within the mycelium of several Phytophthora infestans isolates.
- This finding was achieved through the application of Arbitrary Primed PCR (APCR) and monozoospore plating, confirming the coexistence of distinct mtDNA haplotypes at the cellular level.
Impact:
- Provides novel insights into the mitochondrial genetic diversity of Phytophthora infestans.
- May influence strategies for tracking pathogen populations and understanding disease dynamics.
- Highlights the utility of APCR and monozoospore plating in studying fungal genetics.
Related Concept Videos
01:27Diversity of Protists II
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
01:15Diversity of Protists I
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
00:58Symbiosis
Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...
01:28Toxoplasmosis
Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...
01:09Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
01:22American Trypanosomiasis
Chagas disease, or American trypanosomiasis, is a vector-borne parasitic infection caused by Trypanosoma cruzi, a flagellated protozoan (kinetoplastid) of the family Trypanosomatidae. The disease is endemic in Latin America, although cases are increasingly reported worldwide due to human migration. Transmission most commonly occurs when feces of infected triatomine bugs contaminate bite wounds or mucosal surfaces; additional routes include congenital, transfusional, transplant-related, and oral...