Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling.

Science advances·2026

Same author

Casein kinase 1δ/ε inhibition suppresses CLL proliferation through cell-intrinsic and microenvironmental mechanisms.

HemaSphere·2026

Same author

Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.

Nature neuroscience·2026

Same author

Prevention of transgene silencing during human pluripotent stem cell differentiation.

Cell stem cell·2026

Same author

A class III ligand oscillates between internal and terminal binding modes as it engages with the Dishevelled PDZ domain.

Structure (London, England : 1993)·2025

Same author

Development and Discovery of a Selective Degrader of Casein Kinases 1 δ/ε.

Journal of medicinal chemistry·2024

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Derivation of mouse embryonic stem cells.

Vítezslav Bryja¹, Sonia Bonilla, Ernest Arenas

¹Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden.

Nature Protocols

|May 10, 2007

Summary

This study presents an efficient protocol for deriving mouse embryonic stem cells (mESCs) from blastocysts using specific feeders and media. The method offers high efficiency and simplicity for establishing mESC lines in laboratories.

More Related Videos

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Related Experiment Videos

Last Updated: Jul 15, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

Area of Science:

Developmental Biology
Stem Cell Biology
Genetics

Background:

Derivation of mouse embryonic stem cells (mESCs) is crucial for developmental biology research.
Existing protocols for mESC derivation can be complex and vary in efficiency.
Standardization of mESC derivation protocols is needed for reproducibility.

Purpose of the Study:

To describe a simple, efficient, and reproducible protocol for deriving germline chimera-competent mouse embryonic stem cells (mESCs).
To provide a reliable starting point for laboratories establishing mESC derivation.
To facilitate further optimization for improved efficiency or adaptation to new applications.

Main Methods:

Utilized embryonic day 3.5 (E3.5) mouse blastocysts.
Employed early-passage mouse embryonic fibroblast (MEF) feeders.
Alternated between fetal bovine serum (FBS)-containing and serum replacement (SR)-containing media.

Main Results:

Achieved approximately 50% efficiency in mESC line derivation (50 lines from 96 blastocysts).
All reagents used are commercially available.
The protocol is technically simple and highly efficient compared to existing methods.

Conclusions:

The described protocol offers a straightforward and effective method for mESC derivation.
This protocol is suitable for laboratories initiating mESC line establishment.
The method's efficiency and simplicity make it a valuable tool for stem cell research.