Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Method development of efficient protein extraction in bone tissue for proteome analysis.

Xiaogang Jiang1, Mingliang Ye, Xinning Jiang

  • 1National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Journal of Proteome Research
|May 10, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Hydrological seasonality mediates scale-dependent land use effects on riverine microplastic pollution" [Water Research 301 (2026) 126043].

Water research·2026
Same author

Hydrological seasonality mediates scale-dependent land use effects on riverine microplastic pollution.

Water research·2026
Same author

An integrated microbiome-drug interaction and bioinformatics approach identifies active deglycosylated and glycosidic chain metabolites of Platycodin D targeting PTPN2/HIF1A in acute lung injury.

Biology direct·2026
Same author

Cellular Metabolomics Reveals Hepatoprotective Mechanisms of Platycoside Metabolites Against Acetaminophen-Induced Liver Injury.

Chemistry & biodiversity·2026
Same author

Integrative Transcriptomics and Machine Learning Reveal the Association of CBX4 with Inflammation in Ulcerative Colitis as a Potential Epigenetic Regulator.

Biomedicines·2026
Same author

Acetoacetate-preconditioned adipose stem cells promote burn wound healing through enhanced cellular retention and paracrine signaling.

Burns : journal of the International Society for Burn Injuries·2026

Developing an efficient bone protein extraction method is crucial for understanding bone diseases. This study presents a novel hydrochloric acid (HCl) demineralization technique, significantly improving bone proteome analysis and identifying numerous bone proteins.

Area of Science:

  • Biochemistry
  • Proteomics
  • Biomaterials Science

Background:

  • Bone proteome exploration is vital for understanding bone physiology and pathology.
  • Conventional protein extraction methods for soft tissues are ineffective for bone tissue.
  • Efficient protein extraction method development is critical for bone proteome analysis.

Purpose of the Study:

  • To develop an efficient protein extraction method for large-scale bone proteome analysis.
  • To improve the efficiency of protein extraction from bone tissue.
  • To facilitate comprehensive analysis of the bone proteome.

Main Methods:

  • Bone tissue demineralization using hydrochloric acid (HCl).
  • Sequential protein extraction using three different lysis buffers.

Related Experiment Videos

  • Collection and analysis of proteins from both lysis buffers and the demineralized HCl solution.
  • Automated 2D-LC-MS/MS analysis for peptide and protein identification.

    • Main Results:

    • Significant improvement in protein extraction efficiency using HCl demineralization.
    • Identification of 6202 unique peptides corresponding to 2479 unique proteins.
    • Detection of over 40 bone-specific proteins and 15 potential protein biomarkers.
    • Demonstration of a broad diversity in protein identity and function.

    Conclusions:

    • The developed HCl-based sequential extraction method is highly efficient for comprehensive bone proteome analysis.
    • This method represents the first large-scale proteomic study of bone tissue.
    • The findings may aid in elucidating the mechanisms underlying bone diseases.