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Updated: Jul 15, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using
Ravindra Kumar1, Afaf El-Sagheer, John Tumpane
1School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
Abstract:
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.

