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Improved bread-baking process using Saccharomyces cerevisiae displayed with engineered cyclodextrin

Jae-Hoon Shim¹, Nam-Seok Seo, Sun-Ah Roh

¹Center for Agricultural Biomaterials, School of Agricultural Biotechnology, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Korea.

Journal of Agricultural and Food Chemistry

|May 10, 2007

Summary

A novel enzyme, cyclodextrin glucanotransferase (CGTase), displayed on yeast cells enhances bread and rice cake volume and reduces staling. This yeast-based enzyme system improves baking by efficiently converting starch for fermentation.

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Area of Science:

Biotechnology
Food Science
Enzymology

Background:

Cyclodextrin glucanotransferase (CGTase) is an enzyme that modifies starch.
Engineered CGTase exhibits enhanced starch hydrolysis with reduced cyclodextrin formation.
Cell surface display of enzymes on Saccharomyces cerevisiae offers potential for industrial applications.

Purpose of the Study:

To develop a bread-baking process using cell surface-displayed CGTase.
To evaluate the enzymatic activity and impact on baking quality.
To assess the stability and fermentation efficiency of the engineered yeast.

Main Methods:

Engineering CGTase for enhanced hydrolyzing activity and reduced cyclodextrin formation.
Displaying CGTase on the cell surface of Saccharomyces cerevisiae.
Evaluating bread and rice cake volume, retrogradation, and yeast viability.

Main Results:

Yeast displaying CGTase showed starch-hydrolyzing activity at optimal pH and temperature.
Bread and rice cake volumes increased significantly (20% and 45%) with no detectable cyclodextrin.
Reduced retrogradation rates and improved yeast viability during freeze-thaw cycles were observed.

Conclusions:

Cell surface-displayed CGTase effectively hydrolyzes starch into fermentable sugars for yeast.
This enzyme display system enhances baking quality, including increased volume and reduced staling.
Displaying antistaling enzymes on yeast surfaces presents a promising strategy for improving baking processes.