Related Experiment Video
Updated: Jul 15, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Phylogenetic relationships of German heavy draught horse breeds inferred from mitochondrial DNA D-loop variation
K S Aberle1, H Hamann, C Drögemüller
1Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Hannover, Germany.
Abstract:
We analysed a 610-bp mitochondrial (mt)DNA D-loop fragment in a sample of German draught horse breeds and compared the polymorphic sites with sequences from Arabian, Hanoverian, Exmoor, Icelandic, Sorraia and Przewalski's Horses as well as with Suffolk, Shire and Belgian horses. In a total of 65 horses, 70 polymorphic sites representing 47 haplotypes were observed. The average percentage of polymorphic sites was 11.5% for the mtDNA fragment analysed. In the nine different draught horse breeds including South German, Mecklenburg, Saxon Thuringa coldblood, Rhenisch German, Schleswig Draught Horse, Black Forest Horse, Shire, Suffolk and Belgian, 61 polymorphic sites and 24 haplotypes were found. The phylogenetic analysis failed to show monophyletic groups for the draught horses. The analysis indicated that the draught horse populations investigated consist of diverse genetic groups with respect to their maternal lineage.
Related Concept Videos
Animal Mitochondrial Genetics
Evolutionary Relationships through Genome Comparisons
Microbial Phylogeny
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Incomplete Dominance
Pedigree Analysis

