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[Analysis of full-length molecule of human lipopolysaccharide responsed gene and its expression in four cell lines]
Yuan Bi1, Wen-jing Luo, Li-chao Hou
1Department of Occupational and Environmental Health, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To clone full-length human lipopolysaccharide responsive gene(hlrp) and predict its function by bioinformatics analysis; to observe full-length hlrp protein and quantify its relative gene expression in four cell lines.
Methods:
Total RNA was extracted from LPS-stimulated human embryonic kidney cells HEK293 and the full-length hlrp was obtained by RT-PCR. Function of hlrp was predicted by bioinformatics analysis with Internet and GenBank database. Expression full-length hlrp protein in HEK293, HepG2, HeLa and PDC was observed by laser scanning confocal fluorescence microscope and compared.
Results:
Full-length hlrp of 2 045 bp was amplified and sequenced. Leucine zipper was found in the hlrp series that may have an important function. hlrp gene have been mapped to a particular chromosome location in Xp22.2. Laser scanning confocal fluorescence microscope showed hlrp protein was expressed in the cell lines (HEK293, HepG2, HeLa and PDC).
Conclusion:
hlrp has been successfully cloned and its function has been predicted. Expression of hlrp has been detected in 4 cell lines. Present result would provide data for the further study of hlrp.
