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Updated: Jul 15, 2026
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
[Analysis of Foxp3+ CD4+ CD25+ regulatory cells in peripheral blood of patients with SLE]
Hong-gang Wei1, Bei Cai, Lan-lan Wang
1Clinical Immunological Laboratory, West China Hospital of Sichuan University, Chengdu 610041, China.
Aim:
To investigate the expression of GITR on T cells and the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood of patients with SLE.
Methods:
The expression of GITR and Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood was determined by flow cytometry(FCM).
Results:
Compared with health control, the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in peripheral blood from SLE patients was lower (P<0.05), but there is no difference in the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells between active and inactive SLE patients (P>0.05). GITR expression on both CD3(+) CD4(+) T cells and CD3(+) CD8(+) T cells of SLE patients increased significantly (P>0.05). With the development of SLE activity, GITR expression didn't change significantly on both CD3(+) CD4(+) T cells (P>0.05) and CD3(+) CD8(+) T cells (P>0.05).
Conclusion:
The expression abnormality of both Foxp3(+) CD4(+) CD25(+) regulatory cells and GITR may play important role in the imbalance of immune homeostasis in SLE.
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