Structural organization of the Corynebacterium glutamicum plasmid pCG100
Related Experiment Video
Updated: Sep 13, 2026

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Published on: June 28, 2018
1Laboratoire de Génétique et Physiologie Microbienne, CNRS UA 256, Université de Rennes I, France.
Abstract:
pCG100, a 3 kb cryptic plasmid of Corynebacterium glutamicum ATCC 13058, probably identical with pSR1 from C. glutamicum ATCC 19223, was characterized. The minimum region for autonomous replication was shown to be contained on a 1.9 kb BglII-NcoI fragment; a 380 bp HindIII-SphI fragment can replicate in the presence of the parental plasmid, which presumably provides a trans-acting replication factor. Derivatives of pCG100 are able to replicate in several Corynebacterium, Brevibacterium and Arthrobacter strains. pCG100 is compatible with pBL1, a cryptic plasmid of Brevibacterium lactofermentum. Shuttle plasmid vectors, containing the kanamycin-resistance gene from Tn903 or from Streptococcus faecalis as selectable markers and the AmpR, TetR or lacZ alpha genes for insertional inactivation, were constructed using the minimum replication fragment of pCG100.
Related Concept Videos
Prokaryotic Cells
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Prokaryotic Cells
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins.
Prokaryotic Gene Structure and Organization
Plasmids
Bacterial Phylum Actinobacteria

