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Lysogeny in Leuconostoc oenos.

E K Arendt1, A Lonvaud, W P Hammes

  • 1Institut für Lebensmitteltechnologie, Universität Hohenheim, Stuttgart, Federal Republic of Germany.

Journal of General Microbiology
|September 1, 1991
PubMed
Summary

Researchers induced lysogenic bacteriophages in Leuconostoc oenos strains using mitomycin C. Analysis revealed close relationships among the phages and phage-related sequences within the host bacterial DNA.

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Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • Leuconostoc oenos is a species of lactic acid bacteria.
  • Lysogenic bacteriophages are viruses that can integrate their genetic material into the host bacterium's genome.
  • Mitomycin C is an antibiotic known to induce prophage in lysogenic bacteria.

Purpose of the Study:

  • To investigate the presence and characteristics of lysogenic bacteriophages in Leuconostoc oenos.
  • To determine the relationships between induced phages and their lysogenic hosts.

Main Methods:

  • Induction of prophages using mitomycin C.
  • Determination of lysis curves and isolation of indicator strains.
  • Characterization of phages via electron microscopy, SDS-PAGE, N-terminal amino acid sequencing, and DNA analysis (restriction patterns, cross-hybridization).

Main Results:

  • Nineteen out of thirty Leuconostoc oenos strains yielded inducible phages.
  • Seventeen phages had identifiable indicator strains.
  • Detailed characterization of five phages revealed a very close relationship among them.
  • Hybridization experiments confirmed the presence of phage-related sequences in the lysogenic bacterial DNA.

Conclusions:

  • Leuconostoc oenos harbors inducible temperate bacteriophages.
  • The induced phages exhibit significant genetic relatedness.
  • Phage-host DNA interactions are evident in lysogenic Leuconostoc oenos strains.

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