Related Experiment Video
Updated: Jul 15, 2026
09:49
Experimental Model to Evaluate Resolution of Pneumonia
Published on: February 17, 2023
PNA FISH: present and future impact on patient management
1University of Maryland, Division of Infectious Diseases, Baltimore, MD 21201, USA. gforrest@medicine.umaryland.edu
Expert Review of Molecular Diagnostics
|May 11, 2007
Summary
Peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) offers rapid, accurate results for positive blood cultures. This technology improves patient outcomes and reduces antimicrobial resistance, providing significant cost savings for hospitals.
Area of Science:
- Clinical microbiology
- Molecular diagnostics
- Antimicrobial stewardship
Background:
- Antimicrobial therapy errors lead to poor patient outcomes and resistance.
- Rapid diagnostics are crucial for timely and appropriate treatment decisions.
Purpose of the Study:
- To review clinical data supporting Peptide Nucleic Acid (PNA) fluorescence in situ hybridization (FISH) technology.
- To highlight the impact of PNA FISH on patient management and clinical guidelines.
Main Methods:
- Review of clinical studies evaluating PNA FISH for bloodstream infections.
- Comparison of PNA FISH with conventional methods and real-time PCR.
Main Results:
- PNA FISH provides highly sensitive and specific results within 3 hours of positive blood culture.
- The technology is simpler to implement than real-time PCR, offering significant cost savings.
- Early and targeted therapeutics are enabled by rapid PNA FISH reporting.
Conclusions:
- PNA FISH is a valuable tool for microbiology laboratories, improving patient care.
- This technology supports antimicrobial stewardship and combats antimicrobial resistance.
- PNA FISH is poised to influence future clinical guidelines and laboratory practices.
Related Concept Videos
01:30Pneumonia V: Nursing management and Prevention
Nursing management of pneumonia involves promoting airway patency, facilitating rest and conserving energy, encouraging fluid intake, maintaining nutrition, and educating patients.
The nurse must practice strict medical asepsis and adhere to infection control guidelines to minimize healthcare-associated infections.
Enhance airway patency
Position the patient correctly to facilitate drainage of the affected lung segments. Manual or mechanical percussion and vibration can also be employed.
The nurse must practice strict medical asepsis and adhere to infection control guidelines to minimize healthcare-associated infections.
Enhance airway patency
Position the patient correctly to facilitate drainage of the affected lung segments. Manual or mechanical percussion and vibration can also be employed.
01:28Pneumonia IV: Management
The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
01:30Pneumonia III: Complications and Assessment
Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
01:30Pneumonia I: Introduction
Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
01:29Pneumonia I: Introduction
Pneumonia is an infection of the lower respiratory tract that leads to inflammation of the lung parenchyma, often resulting in the accumulation of inflammatory exudate in the alveoli and airways. Unlike the watery, low-protein fluid exudate in pulmonary edema, the exudate in this case is a thick fluid rich in immune cells, proteins, and debris produced during infection and inflammation.This impairs gas exchange and can lead to consolidation of lung tissue. The infection may be caused by a...
01:27Pulmonary Embolism III: Nursing Management
A pulmonary embolism occurs when a thrombus, amniotic fluid, tumor tissue, fat, or air embolus blocks one or more pulmonary arteries. Effective nursing management and patient education are crucial for improving outcomes and preventing recurrence.Nursing management starts with obtaining a comprehensive patient history, particularly noting any history of deep vein thrombosis (DVT). Assess for clinical manifestations, including dyspnea, chest pain, crackles, heart murmurs, and signs of right-sided...