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Published on: August 23, 2016
In vitro host range of the Hz-1 nonoccluded virus in insect cell lines
Arthur H McIntosh1, James J Grasela, Carlo M Ignoffo
1Agricultural Research Service, Biological Control of Insects Research Laboratory, United States Department of Agriculture, 1503 South Providence Road, Research Park, Columbia, MO 65203-3535, USA. mcintosha@missouri.edu
In Vitro Cellular & Developmental Biology. Animal
|May 12, 2007
Summary
This study found that only Lepidopteran insect cell lines effectively replicate the Hz-1 virus. Specific cell lines like TN-CL1 and BCIRL-HZ-AM1 showed the highest virus titers, indicating their suitability for Hz-1 replication.
Area of Science:
- Insect cell line research
- Virology
- Molecular biology
Background:
- The nonoccluded virus Hz-1's replication capabilities across various insect cell lines are not fully understood.
- Identifying suitable insect cell lines is crucial for baculovirus research and applications.
Purpose of the Study:
- To determine the in vitro host range of the Hz-1 virus across different insect orders.
- To identify specific insect cell lines that efficiently support Hz-1 replication.
Main Methods:
- Testing 13 insect cell lines from 4 orders (Lepidoptera, Coleoptera, Diptera, Homoptera) for Hz-1 replication.
- Measuring virus titers using tissue culture infective dose 50 (TCID50)/ml.
- Performing DNA transfection experiments with Hz-1 and a recombinant baculovirus.
Main Results:
- Only Lepidopteran cell lines supported Hz-1 replication, with TN-CL1 and BCIRL-HZ-AM1 yielding the highest titers (2.4 x 10^8 and 2.0 x 10^8 TCID50/ml).
- The codling moth cell line CP-169 did not support Hz-1 replication or expression of a recombinant baculovirus.
- Hz-1 demonstrated rapid replication in BCIRL-HZ-AM1 cells, peaking at 1.0 x 10^8 TCID50/ml at 56 hours postinoculation.
Conclusions:
- Lepidopteran cell lines, particularly TN-CL1 and BCIRL-HZ-AM1, are optimal for Hz-1 virus replication in vitro.
- Hz-1 exhibits a broad in vitro host spectrum compared to other baculoviruses.
- CP-169 cells are unsuitable for Hz-1 replication and baculovirus gene expression studies.
