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Updated: Jul 15, 2026

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
Published on: February 15, 2022
[Expression of origin recognition complex 1 gene in DNA replication of vascularsmooth muscle cells]
Ming-Hong Jiang1, Mao-Qin Shu, Yue-Long Qin
1Department of Cardiology, South-west Hospital, Third Military Medical University, Chongqing 400038, China.
Objective:
To explore the expression of origin recognition complex 1 (ORC1) during the DNA replication of vascular muscle cells (VSMC).
Methods:
VSMC of thoracic aorta in rats were obtained by the adherence method of tissue culture. The cell synchrony was obtained by the method of double-thymidine block, colchicine treatment and serum starvation. The expression of ORC1 mRNA at different cell cycles of VSMC was determined by RT-PCR and the protein expression of ORC1 was analyzed by Western blot.
Results:
Cultured VSMC were identified by light microscope and immunocytochemistry. Significant expression of ORC1 mRNA and protein in a quiescent stage of VSMC were not observed. Upon synchronization, the expression of ORC1 mRNA was significantly higher at G(1)/S phase of VSMC than that at S and G(2)/M phases. The expression of ORC1 protein followed same changes as the ORC1 mRNA expression at different stages of cell cycles.
Conclusion:
ORC1 may be an important regulatory factor at the initiation of proliferative process of VSMC.
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