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Gene polymorphisms of the macrophage migration inhibitory factor and acute pancreatitis

Rohit Makhija1, Andrew Kingsnorth, Andrew Demaine

  • 1Eastern Deanery, United Kingdom. r_makhija99@hotmail.com

JOP : Journal of the Pancreas
|May 15, 2007
PubMed
Abstract

Insights

The -173 C allele of macrophage migration inhibitory factor (MIF) is overexpressed in acute pancreatitis patients. Further research is needed to confirm this association and explore population genetics of MIF polymorphisms.

Area of Science:

  • Genetics
  • Immunology
  • Gastroenterology

Background:

  • Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine implicated in acute pancreatitis pathogenesis.
  • MIF levels are elevated in human pancreatitis and animal models.
  • Genetic polymorphisms in the MIF gene promoter can influence its expression.

Purpose of the Study:

  • To investigate the association between MIF gene polymorphisms and acute pancreatitis in a UK population.
  • To analyze the distribution of specific MIF polymorphisms (-173 G>C and -794 (CATT)n) in patients with acute pancreatitis and healthy controls.

Main Methods:

  • Genotyping of 164 acute pancreatitis patients and 197 healthy controls.
  • Analysis of the -173 G to C single nucleotide polymorphism using RFLP.
  • Analysis of the -794 (CATT)n microsatellite using PCR and PAGE.

Main Results:

  • The -794 (CATT)n microsatellite showed no significant difference between patients and controls.
  • A trend towards reduced frequency of the -173 GG genotype was observed in patients (P=0.056).
  • The -173 C allele was significantly overexpressed in acute pancreatitis patients (P=0.025).

Conclusions:

  • The -173 C allele of MIF is associated with an increased risk of acute pancreatitis.
  • Further studies are required to validate these findings and explore the population genetics of MIF microsatellite alleles.
  • No significant differences were found in subgroups based on pancreatitis severity or etiology.

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