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Related Experiment Video

Updated: Jul 15, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

SPP1 polymorphisms associated with HBV clearance and HCC occurrence.

Hyoung Doo Shin¹, Byung Lae Park, Hyun Sub Cheong

¹Department of Genetic Epidemiology, SNP Genetics, Inc., Rm 1407, 14th floor, Complex B, WooLim Lion's Valley, 371-28, Gasan-Dong, Geumcheon-Gu, Seoul, 153-803, Republic of Korea.

International Journal of Epidemiology

|May 15, 2007

Summary

Genetic variations in Secreted Phosphoprotein-1 (SPP1) are linked to hepatitis B virus (HBV) clearance and the onset of hepatocellular carcinoma (HCC). SPP1 polymorphisms may influence host susceptibility to HBV infection outcomes and HCC development.

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Last Updated: Jul 15, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

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Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

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Published on: October 20, 2021

Area of Science:

Genetics
Hepatology
Immunology

Background:

Secreted Phosphoprotein-1 (SPP1) is a phosphoprotein overexpressed in metastatic hepatocellular carcinoma (HCC).
SPP1's role in metastatic HCC suggests potential as a diagnostic marker and therapeutic target.
Investigating SPP1 genetic polymorphisms may reveal host genetic factors influencing HBV clearance and HCC.

Purpose of the Study:

To investigate the association between SPP1 genetic polymorphisms and hepatitis B virus (HBV) clearance.
To determine if SPP1 polymorphisms are linked to the occurrence and progression of hepatocellular carcinoma (HCC).
To explore SPP1 as a candidate gene in host genetic studies of HBV infection and HCC.

Main Methods:

Genotyping of five SPP1 genetic variants using TaqMan assay.
Association analysis of SPP1 polymorphisms with HBV clearance in a Korean cohort (n=1,069).
Cox relative hazard analysis to assess the impact of SPP1 variants on HCC occurrence age.

Main Results:

A common SPP1 haplotype (SPP1-ht2) was significantly associated with HBV clearance.
SPP1-ht2 genotype frequency was higher in chronic carriers (CC) than spontaneously recovered (SR) individuals (OR=1.44, P=0.006).
SPP1-ht2 and a specific variant (-1,800G>T) were associated with earlier HCC onset in chronic hepatitis patients (RH=1.85 for both).

Conclusions:

SPP1 genetic polymorphisms appear to be significant host genetic factors influencing HBV clearance.
SPP1 variants are associated with the age of HCC occurrence among patients with chronic hepatitis B.
These findings highlight SPP1's potential role in the pathogenesis of HBV infection outcomes and HCC development.