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Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

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High-Resolution Fluorespirometry to Assess Dynamic Changes in Mitochondrial Membrane Potential in Human Immune Cells
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Evaluating mitochondrial membrane potential in cells.

Giancarlo Solaini1, Gianluca Sgarbi, Giorgio Lenaz

  • 1Dipartimento di Biochimica, Università di Bologna, Via Irnerio 48, Bologna, 40126, Italy. giancarlo.solaini@unibo.it

Bioscience Reports
|May 15, 2007
PubMed
Summary

This review covers how cationic fluorescent probes monitor mitochondrial membrane potential, crucial for understanding cell metabolism and disease. It offers practical tips for accurate fluorescence measurements in biological research.

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Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
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Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Permeant cationic fluorescent probes are essential for assessing mitochondrial transmembrane potential.
  • Monitoring mitochondrial membrane potential provides insights into cellular energy metabolism and its dysfunction in pathological conditions.

Purpose of the Study:

  • To review current analytical methods for measuring mitochondrial membrane potential using fluorescent probes.
  • To provide practical guidance for data acquisition and analysis in fluorescence-based studies.
  • To discuss the strengths and weaknesses of various fluorescence techniques.

Main Methods:

  • Review of literature on fluorescent probes for mitochondrial membrane potential.
  • Analysis of fluorescence microscopy and spectroscopy techniques.
  • Discussion of principles of fluorescence emission, quenching, and quantum yield.

Main Results:

  • Fluorescent probes offer valuable insights into cellular energy metabolism.
  • Environmental factors and instrument limitations can significantly affect fluorescence signals.
  • Various techniques have distinct advantages and disadvantages for analyzing mitochondrial membrane potential.

Conclusions:

  • Accurate measurement of mitochondrial membrane potential using fluorescent probes requires careful consideration of probe properties and instrument limitations.
  • Practical tips for data acquisition and analysis are crucial for reliable results.
  • Understanding the strengths and weaknesses of different techniques aids in selecting appropriate methods for specific research questions.