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Related Experiment Videos

Acute hydrodynamic forces and apoptosis: a complex question.

Mike Mollet1, Ruben Godoy-Silva, Claudia Berdugo

  • 1Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W 19th Ave., Columbus, Ohio 43210, USA.

Biotechnology and Bioengineering
|May 15, 2007
PubMed
Summary
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This study developed a flow device to apply hydrodynamic forces to cells. Results show minimal apoptosis induction by these forces, with increased necrosis in serum-free suspension cultures.

Area of Science:

  • Biophysics
  • Cell Biology
  • Biotechnology

Background:

  • Hydrodynamic forces can impact cell viability.
  • Understanding cell responses to mechanical stress is crucial for biotechnology.
  • Previous studies established energy dissipation rates (EDR) for cell lysis.

Purpose of the Study:

  • To investigate apoptosis induction by hydrodynamic forces in Chinese Hamster Ovary (CHO) cells.
  • To determine the influence of culture media and history on apoptosis and necrosis.
  • To assess the role of the Bcl-2 gene in cellular response to hydrodynamic stress.

Main Methods:

  • Development and modeling of a second-generation flow contraction device.
  • Application of controlled hydrodynamic forces to wild-type CHO-K1 and CHO-bcl-2 cells.

Related Experiment Videos

  • Comparison of cell responses across different culture conditions (T-flask vs. suspension, serum vs. serum-free media).

    • Main Results:

    • The flow device and simulations align with prior EDR findings for cell lysis.
    • Sub-lysis EDR levels (<10^8 Wxm^-3) induced minimal apoptosis in CHO cells.
    • Suspended cells in serum-free media showed significantly increased necrosis but not apoptosis compared to controls.
    • Bcl-2 expression had a negligible effect on apoptosis induction due to low baseline apoptosis rates.

    Conclusions:

    • Acute hydrodynamic forces at sub-lysis levels induce limited apoptosis in CHO cells.
    • Serum-free suspension culture conditions increase necrosis under hydrodynamic stress.
    • The Bcl-2 gene offers no significant protective effect against hydrodynamic-induced apoptosis in this model.
    • The developed flow device provides a reliable platform for studying cellular responses to mechanical forces.